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Based on the chemical framework of the analyte, the molecules are retarded in the column stationary stage as a result of unique intermolecular interactions amongst the analyte along with the packing materials around the column.Because air is not an excellent, negative heat conductor, it truly is less helpful in managing the column temperature in au
. The working pump as well as the equilibrating pump Just about every Have got a piston whose forwards and backwards motion maintains a constant movement level of nearly quite a few mL/min and delivers the high output tension required to drive the mobile section from the chromatographic column.. HPLC separation of a combination of flavonoids with U
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